opentrons-heater-shaker
Opentrons Heater-Shaker Module - temperature control (37-95°C) with orbital mixing (200-3000 rpm) for cell culture, enzymatic reactions, and sample preparation requiring simultaneous heating and agitation
Security Assessment
About opentrons-heater-shaker
The Opentrons Heater-Shaker Module skill is designed to automate protocols that require simultaneous temperature control and orbital mixing. It offers precise temperature control (37-95°C) along with adjustable shaking speeds (200-3000 rpm), enabling the automation of temperature-sensitive processes. This skill effectively replaces manual incubation transfers and provides a hands-off solution for protocols that require both heating and mixing, ensuring reproducibility and consistency in your experiments. By integrating temperature control directly on the deck, the Heater-Shaker module streamlines workflows and reduces the risk of human error.
Main features of the Heater-Shaker module include customizable temperature settings within the 37-95°C range and adjustable shaking speeds for precise control over agitation. It allows for both blocking and non-blocking operations, depending on whether you need the protocol to pause while reaching the target temperature or speed. The module supports various labware adapters, allowing you to use different types of plates, such as flat-bottom plates, PCR plates, and deep-well plates. The system also features a latch control to secure labware during shaking operations, ensuring reliable performance in automated setups.
This skill is perfect for researchers and labs working with cell culture, enzymatic reactions, or any experiment requiring precise temperature and agitation control. Ideal use cases include bacterial transformations, ELISA washing steps, hybridization reactions, and protocols requiring bead resuspension with controlled agitation. The module is compatible with both the Opentrons Flex and OT-2 platforms, offering flexibility in deck configurations and compatibility with a wide range of labware setups.
FAQ
How do I load the Heater-Shaker module?
You can load the Heater-Shaker module using `protocol.load_module('heaterShakerModuleV1', location='slot')` where 'slot' is the deck position where you want to place the module.
What labware can be used with the Heater-Shaker?
The Heater-Shaker is compatible with various labware types, including 96-well flat-bottom plates, PCR plates, and deep-well plates. You can use different adapters to accommodate different plate types.
Can I use the Heater-Shaker module for just temperature control?
No, if you only need temperature control, it is recommended to use the Temperature Module instead, as the Heater-Shaker is designed for both heating and mixing.
Which Opentrons platforms are compatible with the Heater-Shaker module?
The Heater-Shaker module is compatible with both the Opentrons Flex and OT-2 platforms. Each platform has specific slot configurations and adjacency restrictions for loading the module.
Are there any limitations to using the Heater-Shaker module?
The Heater-Shaker module is limited to a temperature range of 37-95°C, and should not be used for protocols requiring temperatures outside of this range. It is also not suitable for tasks that only require mixing or only require heating.
Install opentrons-heater-shaker
Quick Setup:
- Copy the skill folder to
.claude/skills/ - Claude will automatically detect and use the skill
Repository
jkitchin/skillz