opentrons-absorbance-reader

The opentrons-absorbance-reader skill enables control of the Opentrons Absorbance Plate Reader Module on the Opentrons Flex platform. This module is an on-deck microplate spectrophotometer designed to measure light absorbance directly within automated workflows, eliminating the need for manual plate transfers to a separate reader. By integrating absorbance measurements into robotic protocols, users can perform mid-protocol readings, collect kinetic data, and make real-time decisions without interrupting automation. This significantly improves workflow efficiency and reduces handling errors in plate-based assays.

The module supports absorbance measurements in standard 96-well plates at up to six wavelengths simultaneously, with available wavelengths of 450 nm, 562 nm, 600 nm, and 650 nm. It provides automated lid control using the Flex gripper, including methods to open, close, and check lid status. Initialization allows configuration of measurement parameters such as mode, selected wavelengths, and optional reference wavelength. Read operations can optionally export data as CSV files. The module must be loaded in column 3 (A3–D3), and column 4 is reserved as a staging area for lid storage. The API requires version 2.21 or later and is compatible exclusively with the Opentrons Flex robot.

This skill is intended for laboratory automation engineers, researchers, and assay developers running ELISA, OD600 cell growth measurements, colorimetric assays, and kinetic absorbance experiments. It is particularly valuable in workflows requiring multi-wavelength data collection or absorbance-based decision points within automated biochemical protocols.